Abstract

EXTRACTION, PHYTOCHEMICAL ANALYSIS AND STUDY OF PHARMACOLOGICAL POTENTIAL OF INULA RECEMOSA EXTRACT

Ashutosh Dubey*, Jitendra Kumar Malik, Gyan Singh

P.K. University, Thanra, Shivpuri, M.P.

ABSTRACT

The present study was undertaken to evaluate the extraction, phytochemical constituents, and pharmacological potential of the ethanolic extract of Inula racemosa. The plant extract was prepared using ethanol, yielding 10.52% w/w, indicating effective extraction of bioactive compounds. Preliminary phytochemical screening revealed the presence of important secondary metabolites such as flavonoids, phenols, glycosides, saponins, proteins, and carbohydrates, while alkaloids, tannins, diterpenes, and sterols were absent. Quantitative analysis showed appreciable amounts of total phenolic (0.652 mg/100 mg) and flavonoid content (0.741 mg/100 mg), suggesting significant antioxidant potential. The pharmacological evaluation demonstrated that the extract exhibited dose-dependent anti-inflammatory activity, though less potent than diclofenac sodium, with an IC₅₀ value of 114.21 µg/ml. antimicrobial studies showed moderate activity against Streptococcus mutans and Escherichia coli, with increasing zones of inhibition at higher concentrations. Furthermore, the extract displayed notable antidiabetic activity through α-amylase inhibition, with an IC₅₀ value of 98.52 µg/ml compared to acarbose. Overall, the findings indicate that Inula racemosa possesses significant pharmacological properties, which may be attributed to its rich phytochemical composition. Although the activity is comparatively lower than standard drugs, the plant extract offers a promising natural alternative for therapeutic applications. Further studies are required to isolate active constituents and validate its efficacy through in-vivo models.

Keywords: Inula racemosa, Ethanolic extract, Phytochemical screening, Flavonoids, Phenolic compounds, Anti-inflammatory activity, Antimicrobial activity, Antidiabetic activity, IC??, Medicinal plants.