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Abstract

HPTLC ESTIMATION OF BIOACTIVE COMPOUND PRESENT IN SMILAX MACROPHYLLA

Adarsh Kesarwani*1, B.K. Dubey1, Deepak Kumar Basedia1, Sunil Kumar Shah2

1Technocrats Institute of Technology-Pharmacy, Bhopal (M.P.)
2TIT-College of Pharmacy, Bhopal (M.P.)

ABSTRACT

Smilax macrophylla is a medicinal plant traditionally used for the treatment of various ailments due to its rich phytochemical composition. The present study aimed to evaluate the phytochemical profile and to estimate a bioactive flavonoid marker, quercetin, in the hydroalcoholic extract of Smilax macrophylla using High-Performance Thin-Layer Chromatography (HPTLC). The hydroalcoholic extract was prepared and subjected to preliminary phytochemical screening, which revealed the presence of glycosides, flavonoids, phenolic compounds, saponins, diterpenes, and tannins. Thin-layer chromatographic analysis was carried out using toluene:ethyl acetate:formic acid (5:4:1 v/v/v) as the mobile phase, and the presence of quercetin in the extract was confirmed by comparing Rf values with the standard. HPTLC analysis was performed using silica gel 60 F₂₅₄ plates with densitometric scanning at 425 nm. Quantitative estimation showed that the total flavonoid and total phenolic contents of the extract were 0.82 mg/100 mg and 0.37 mg/100 mg of dried extract, respectively. The percentage content of quercetin in the hydroalcoholic extract was found to be 0.75%. The results demonstrate that HPTLC is a simple, precise, and reliable method for the identification and quantification of bioactive compounds in Smilax macrophylla, supporting its standardization and quality control.

Keywords: Smilax macrophylla, HPTLC, Quercetin; Flavonoids, Phytochemical screening, Herbal standardization.


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