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Abstract

QUALITATIVE AND QUANTITATIVE STUDY OF BIOACTIVE COMPOUND USING SPECTROSCOPIC AND CHROMATOGRAPHIC TECHNIQUES

Srashti Sahu*, Jyoti Ahirwar, Durga Kishora, Kuldeep Ganju

Sagar Institute of Pharmacy and Technology (SIPTec), Bhopal (M.P.)

ABSTRACT

The present study aimed to perform qualitative and quantitative analysis of bioactive compounds present in the hydroalcoholic extract of Citrullus lanatus using spectroscopic and chromatographic techniques. The extract was prepared by maceration and evaluated for its physicochemical properties, percentage yield, and phytochemical profile. Preliminary phytochemical screening revealed the presence of carbohydrates, flavonoids, phenols, proteins, and diterpenes. Quantitative estimation indicated total phenolic and flavonoid contents of 0.52 mg/100 mg and 0.87 mg/100 mg, respectively. High-Performance Liquid Chromatography (HPLC) analysis confirmed the presence of quercetin with a retention time of 2.369 minutes and a concentration of 0.211%. These findings demonstrate that Citrullus lanatus is a rich source of natural antioxidants and bioactive compounds, which could be utilized for pharmaceutical and nutraceutical applications.

Keywords: Citrullus lanatus, Phytochemical screening, Quercetin, Spectroscopic analysis, HPLC, Bioactive compounds, Antioxidant potential


[Full Text Article]